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竹荪是一种名贵的食用菌,在人工栽培技术方面,已有不少报告。我们于1988年作了秸秆发酵料栽培竹荪试验,取得了一定效果,现报告如下。(一)菌种筛选①母种培养基筛选:设计六个母种培养基,以PDA 作对照。结果以配方六为最好,1988年4月4日接种,4月25日长满试管(20×200mm)斜面,共21天。②引自上海、广东、湖南、湖北及本厂自野生竹荪分离的长裙竹荪和短裙竹荪六个菌株中,经品种比较试验表明,在相同的培养条件下,以上海农科院食用菌所的长裙竹荪,菌丝生长最快、浓密,在23℃下避光培养,20天可长满斜面。因此,本试验菌种选用该菌株。(二)原种及栽培种制作①原种培养:配方是竹片10%(煮汁)、阔叶树木屑68%、麸皮20%、石膏1%、糖1%,另加蛋白胨5克,含水量65%。常规装瓶、培养、接种。每支母种接3瓶。在自然温度为23～29℃下避光培养,49天长满瓶。②栽培种培养:配方是竹片10%(煮汁)、阔叶树木屑68%、麸皮10%、玉米粉10%、石膏1%、糖1%,含水量65%。常规装瓶、灭菌、接种。每瓶原种接50瓶左右。在自然温度为25～29℃下避光培养,55天长满瓶。
Dictyophora is a valuable edible fungus, in artificial cultivation techniques, there have been many reports. We made a straw fermented material cultivation bamboo sun test in 1988, and achieved certain results, are as follows. (A) strain screening ① mother culture medium screening: design of six mother culture medium, with PDA as a control. The results of the formula six is the best, April 4, 1988 vaccination, April 25 full of test tubes (20 × 200mm) incline, a total of 21 days. (2) Six strains of Dictyophora and Dictyophora were isolated from Shanghai, Guangdong, Hunan, Hubei and our factory. The comparative experiments showed that under the same culture conditions, Hospital edible fungus long skirt Dictyophora, hyphae fastest growing, dense, dark at 23 ℃ culture, 20 days can be covered with beveled. Therefore, the test strain selection of the strain. (B) the original species and cultivars production ① original culture: the recipe is bamboo 10% (cooking juice), 68% of hardwood trees, bran 20%, gypsum 1%, 1% sugar, plus peptone 5 grams, 65%. Routine bottling, cultivation, vaccination. Take 3 bottles of each mother’s seed. In the natural temperature of 23 ~ 29 ℃ dark culture, 49 days long full bottle. ② Cultivation Cultivation: The recipe is bamboo 10% (cooking juice), hardwood sawdust 68%, bran 10%, corn flour 10%, gypsum 1%, sugar 1%, water content 65%. Routine bottling, sterilization, vaccination. Each bottle of original 50 bottles. In the natural temperature of 25 ~ 29 ℃ dark culture, 55 days full bottle.