论文部分内容阅读
目的建立人脊索瘤细胞系,为脊索瘤研究提供实验模型。方法取经病理证实的新鲜脊索瘤手术标本(取自于1例42岁男患者行手术切除后的肿瘤),进行体外原代组织块培养。对存活细胞进行形态学观察、组织化学染色、细胞周期检查、染色体分析、电镜观察、异种移植和体外侵袭实验等。结果建成细胞系CM-319,经近两年的体外培养,已连续传代百余次。其形态学表现、组织化学染色、电镜观察和异种移植等均符合脊索瘤细胞特征。细胞倍增时间为33h,细胞周期测定显示:G1期为55.6%,G2期为21.9%,S期为22.5%,G2/G1为1.90。染色体具有亚三倍体核型。异种移植成瘤率100%,具有侵袭性。结论CM-319是一株人脊索瘤细胞系,可用于对脊索瘤的研究。
Objective To establish human chordoma cell lines and provide experimental models for the study of chordoma. Methods The specimens of fresh chordoma confirmed by pathology (taken from a 42-year-old male undergoing surgical resection) were cultured in primary culture in vitro. Surviving cells were observed morphologically, histochemical staining, cell cycle check, chromosome analysis, electron microscopy, xenograft and in vitro invasion experiments. Results Established cell line CM-319, after nearly two years of in vitro culture, has been passed on for more than 100 times. The morphological features, histochemical staining, electron microscopy and xenotransplantation were consistent with chordoma cell characteristics. The cell doubling time was 33h. The cell cycle assay showed 55.6% in G1 phase, 21.9% in G2 phase, 22.5% in S phase and 1.90 in G2 / G1. Chromosomes have sub-triploid karyotypes. Xenograft tumor formation rate of 100%, with invasive. Conclusion CM-319 is a human chordoma cell line that can be used to study chordoma.