目前吡柔比星广泛用于膀胱灌注治疗膀胱癌,但是其疗效因耐药性会受到一定的影响,目前吡柔比星的作用机制还没得到完全的阐述。本研究通过吡柔比星、siRNA和3-甲基腺嘌呤或羟氯喹处理膀胱癌细胞EJ、J82,采用细胞活力分析仪和流式细胞仪分别检测膀胱癌细胞的增殖和凋亡。处理前后通过免疫印迹法评估自体吞噬情况,同时检测雷帕霉素的磷酸化哺乳动物靶点、p70核糖体蛋白S6激酶和真 Currently pirarubicin is widely used in bladder irrigation bladder cancer treatment, but its efficacy due to drug resistance will be affected to some extent, the current mechanism of pirarubicin has not yet been fully elaborated. In this study, bladder cancer cells EJ and J82 were treated with pirarubicin, siRNA and 3-methyladenine or hydroxychloroquine. The proliferation and apoptosis of bladder cancer cells were detected by cell viability analyzer and flow cytometry respectively. Autophagy was assessed by immunoblotting before and after treatment, while the phosphorylated mammalian target of rapamycin, p70 ribosomal protein S6 kinase and true