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目的探讨胆囊收缩素B(CCK-B)受体在人食管鳞癌(EC)组织及EC旁组织中的表达。方法收集EC组织标本30例和对应癌旁正常食管组织,采用RT-PCR法检测EC和癌旁组织中CCK-B受体mRNA的表达;免疫组化染色法检测CCK-B受体蛋白在EC组织中的表达水平以及与临床分期和病理分级之间的关系。结果 30例EC组织中有27例(90.0%)CCK-B受体mRNA呈阳性,癌旁正常组织呈阴性表达;30例EC组织中CCK-B受体有25例(83.3%)呈阳性表达。EC组织不同病理学分级各组间CCK-B受体阳性表达率经统计学处理,无统计学差异。结论 CCK-B受体的表达是EC发生的早期事件,可用作EC临床诊断的生物学标志。
Objective To investigate the expression of cholecystokinin B (CCK-B) receptor in human esophageal squamous carcinoma (EC) and para-EC tissues. Methods The EC tissues and corresponding normal esophageal tissues were collected from 30 EC samples and the expression of CCK-B receptor mRNA in EC and adjacent tissues was detected by RT-PCR. The expression of CCK-B receptor protein in EC The level of expression in the tissue and its relationship to clinical stage and pathological grade. Results CCK-B receptor mRNA was positive in 27 cases (90.0%) of EC tissues and negative in adjacent normal tissues. The expression of CCK-B receptor was positive in 25 of 30 EC tissues (83.3%) . The positive rate of CCK-B receptor in different pathological grades of EC tissues was statistically analyzed, with no statistical difference. Conclusion The expression of CCK-B receptor is an early event of EC. It can be used as a biomarker for the clinical diagnosis of EC.