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目的:探讨白介素-4(IL-4)、白介素-13(IL-13)以及β2肾上腺素能受体(ADRβ2)基因多态性与我国汉族慢性阻塞性肺病(COPD)发生之间的相关性。方法:本研究采用病例对照性研究,通过聚合酶链反应和限制性片段长度多态性(PCR-RFLP)方法检测106例COPD患者和72例健康对照者IL-4[-589C/T、-33C/T、可变数目串联重复序列(VNTR)]、IL-13(-1111C/T、-2044G/A)以及ADRβ2(-46A/G及-79C/G)的基因多态性,同时以Taq-Man等位基因识别技术检测单倍型频率、基因型以及等位基因频率。结果:COPD组与对照组的ADRβ2-79C/G基因型的频率分布存在显著性差异(P=0.002)。同时,2组间单倍型基因频率(IL4-589C/T:ADRβ2-79C/G以及IL4VNTR:ADRβ2-79C/G)也存在显著性差异(P值分别为0.033与0.001)。结论:ADRβ2-79C/G基因多态性及单倍型可能参与COPD的发病机制。
Objective: To investigate the correlation between IL-4, IL-13 and ADRβ2 gene polymorphisms and the occurrence of chronic obstructive pulmonary disease (COPD) in Han nationality in China . Methods: A case-control study was conducted in this study. The expression of IL-4 [-589C / T, - (-)] in 106 COPD patients and 72 healthy controls were detected by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) 33C / T, VNTR), IL-13 (-1111C / T, -2044G / A) and ADRβ2 (-46A / G and -79C / G) Taq-Man Allele Recognition Technique to Detect Haplotype Frequency, Genotype, and Allele Frequency. Results: There was a significant difference in the frequency distribution of ADRβ2-79C / G genotype between COPD group and control group (P = 0.002). At the same time, haplotype frequencies (IL4-589C / T: ADRβ2-79C / G and IL4VNTR: ADRβ2-79C / G) were also significantly different between the two groups (P values were 0.033 and 0.001, respectively). Conclusion: The ADRβ2-79C / G polymorphism and haplotype may be involved in the pathogenesis of COPD.