在普通易变(晚期发作)性的低丙球蛋白血症(CVH)病人,恶性肿瘤发生率显著升高的原因迄今未能得到解释。在体内具有十分有效的肿瘤清除活性的LAK细胞的功能,在CVH病人受到损伤。这可能(至少是部分地)是肿瘤发生率升高的原因。作者研究了LAK细胞功能降低的机制。为了排除这是由于LAK细胞前身减少所致,从而在应用特异的细胞毒性T细胞前身的基础上,建立了一种有限稀释试验。自外周血分离单个核细胞,由每个圆底微孔150 000细胞开始,作双倍稀释的24个复孔。每孔最终容积 The reason for the significant increase in the incidence of malignancies in a generally variable (late-onset) hypoglycaemia (CVH) patient has so far failed to explain. The function of LAK cells, which have very potent tumor-clearing activity in vivo, is impaired in CVH patients. This may be, at least in part, the cause of an increase in the incidence of tumors. The authors studied the mechanism of LAK cell function decline. In order to rule out this is due to a decrease of precursor of LAK cells, a limited dilution test based on the application of specific cytotoxic T-cell precursors was established. Mononuclear cells were isolated from peripheral blood, starting from 150,000 cells per round bottom well and 24 double wells double-diluted. The final volume of each hole