论文部分内容阅读
以新疆辣椒主栽品种“四平头”（带毒率较高）为试材，切取无菌苗生长点，以ＭＳ为基本培养基，在其上分别添加６－苄基腺嘌呤（６－ＢＡ）、吲哚乙酸（ＩＡＡ）、玉米素（ＺＴ）、细胞激动素（ＫＴ）、赤霉素（ＧＡ３）和萘乙酸（ＮＡＡ）、苯乙酸（ＰＡＡ）以及吲哚丁酸（ＩＢＡ）等生长调节剂进行培养。经筛选８０余种不同激素水平的培养基，其中以ＭＳ＋ＢＡ８＋ＩＡＡ０．０５～１．０较适宜茎尖的培养，ＭＳ＋ＫＴ０．２＋ＧＡ３０．５～５或ＭＳ＋ＺＴ８＋ＧＡ３２～３较适宜芽的伸长。将伸长１ｃｍ以上的芽转入ＭＳ＋ＩＢＡ２或ＭＳ无激素的培养基上利于根的生长，生根后移入土壤即可长成正常植株。成苗脱毒率达１００％。
In this experiment, the growing point of aseptic seedlings was cut with “Sipingtou” (high virulent rate) of pepper cultivars in Xinjiang, MS was taken as the basic medium, and 6-benzyl adenine , IAA, ZT, KT, GA3 and NAA, PAA and IBA, etc. Conditioner for cultivation. After more than 80 different hormone levels were screened, MS + KT0.2 + GA30.5 ~ 5 or MS + ZT8 + GA32 ~ 3 was more suitable for shoot growth with MS + BA8 + IAA 0.05 ~ 1.0. The elongation of more than 1cm buds into MS + IBA2 or MS hormone-free medium on the growth of the root, after rooting into the soil can grow into normal plants. Seedlings off the virus rate of 100%.