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目的：骨髓造血干细胞（ｈｅｍｏｐｏｉｅｔｉｃｓｔｅｍｃｅｌｌ，ＨＳＣ）损伤所致的造血抑制是急性骨髓型放射病死亡的重要原因之一，本实验旨在观察人基因工程重组超氧化物歧化酶（ｒｈＳＯＤ）对ＨＳＣ有无辐射防护作用以及化学修饰对ｒｈＳＯＤ抗辐射作用的影响。方法：实验用照射小鼠的外源性脾结节（ＣＦＵ－Ｓ）形成法。组间比较用ｔ检验。结果：ｒｈＳＯＤ和ＰＥＧ－ＳＯＤ均能明显提高ＣＦＵ－Ｓ形成数，其中ｒｈＳＯＤ在照射前１小时给药效果好，而ＰＥＧ－ＳＯＤ在照射前２～３小时给药作用更为明显。结论：ｒｈＳＯＤ具有对骨髓造血于细胞的辐射防护作用，化学修饰后ＳＯＤ的血浆半衰期延长而使药物作用持续时间延长。
OBJECTIVE: The hematopoietic inhibition induced by bone marrow hematopoietic stem cell (HSC) injury is one of the important causes of death of acute myeloid leukemia. The purpose of this experiment was to observe whether human genetic recombinant superoxide dismutase (rhSOD) Effects of Protective and Chemical Modification on Radiation Resistance of rhSOD. Methods: Exogenous spleen nodules (CFU-S) formation in irradiated mice were used. Comparison between groups using t test. Results: Both rhSOD and PEG-SOD could significantly increase the number of CFU-S formation, of which rhSOD was administered 1 hour before irradiation and PEG-SOD was more effective 2 to 3 hours before irradiation. Conclusion: rhSOD has the radioprotective effect on bone marrow hematopoietic cells. After the chemical modification, the plasma half-life of SOD is prolonged and the duration of drug action is prolonged.