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胰腺癌是一种恶性程度极高,早期难以诊断,预后极差的恶性肿瘤,临床确诊者大多属于晚期癌,5年生存率总体低于5%。我们采用突变体富集聚合酶链反应(PCR)检测胰腺癌细胞系K ras基因点突变,以期为早期诊断胰腺癌提供一种简便、灵敏而有效的检验方法。一、材料与方法1.材料:胰腺癌细
Pancreatic cancer is a kind of malignant tumor with extremely high malignant degree, early diagnosis and poor prognosis. Most of the patients with clinical diagnosis belong to advanced cancer, and the overall 5-year survival rate is lower than 5%. We used mutant-enriched polymerase chain reaction (PCR) to detect K ras gene point mutations in pancreatic cancer cell lines in order to provide a simple, sensitive and effective method for the early diagnosis of pancreatic cancer. First, materials and methods 1. Materials: pancreatic cancer fine