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目的:探讨小鼠单纯疱疹病毒Ⅰ型心肌炎细胞凋亡与TGF-β1表达的关系。方法与结果:通过给BALB/C小鼠腹腔接种单纯疱疹病毒Ⅰ型(HSV-1)诱发病毒性心肌炎(VMC),感染病毒3～35 d,病毒性心肌炎(VMC)检出率为91.67%。应用原位末端标记法(TUNEL)及免疫组化技术检测发现:78.33%感染病毒鼠心肌中发现凋亡细胞,71.67%有TGF-β1的表达,二者之间分布区域一致。结论:细胞凋亡可能参与HSV-1诱导的VMC的发生、发展,而TGF-β1可能参与VMC细胞凋亡的调控。
Objective: To investigate the relationship between the apoptosis of mouse with herpes simplex virus type 1 myocarditis and the expression of TGF-β1. Methods and Results: The detection rate of viral myocarditis (VMC) in VMC was 91.67% by inoculation of herpes simplex virus type 1 (HSV-1) into BALB / C mice intraperitoneally with viral myocarditis (VMC) . TUNEL and immunohistochemistry showed that apoptotic cells were found in 78.33% infected rat myocardium, and 71.67% had TGF-β1 expression. The distribution between the two was consistent. Conclusion: Apoptosis may be involved in the occurrence and development of VMC induced by HSV-1, and TGF-β1 may be involved in the regulation of VMC apoptosis.