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本实验表明,超氧物歧化酶(SOD)普遍存在于葡萄中.供试材料的SOD同工酶酶谱类型基本相同。在田间栽培条件下,7月中旬气温最高时,供试材料SOD同工酶谱带最少,各个种、品种间SOD同工酶谱带数差异甚微.随着日照对数缩短,气温降低,葡萄进入越冬前准备时期,供试材料的SOD同工酶谱带数均明显增加。抗寒力强的种和品种,SOD同工酶谱带数增加的多,SOD同工酶活性也强.嫁接苗SOD同工酶谱带数变化符合上述趋势,而且嫁接苗SOD同工酶谱带数增加情况与砧木自根苗相同,而与嫁穗自根苗不同。砧木自根苗抗寒力比接穗自根苗抗寒力强,砧木对嫁接苗抗寒力的影响也表现在SOD同工酶上.上述结果表明,SOD与葡萄抗寒力有密切关系,入冬前SOD同工酶谱带数增加和酶活性增强是对逐渐降低的温度的反应。
This experiment shows that SOD is commonly found in grapes, SOD isozymes of the tested materials are basically the same type. Under the field cultivation conditions, the SOD isozyme bands of the tested materials were the least at the highest temperature in mid-July, and there was very little difference in the number of SOD isozymes between the varieties and cultivars.As the sunshine logarithm shortened and the temperature decreased, Grape entered the winter before the preparation period, the SOD isozyme bands were significantly increased. Cold-tolerant varieties and varieties, SOD isozyme bands increased more, SOD isozyme activity is also strong. Grape seedlings SOD isozyme bands in line with the above trends, and grafted seedlings SOD isozymes With the increase of the number of rootstocks from the rootstock the same, but different from the rooting shoots. Cold-rooted rootstocks than the rootstock cold-resistant than the self-rooted seedlings cold hardiness, rootstocks on the grafted seedlings cold hardiness is also reflected in the SOD isozymes.The above results show that SOD and grape cold hardiness, before winter Increased SOD isozyme bands and increased enzymatic activity are responses to decreasing temperatures.