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目的:研究低氧条件下血管紧张素Ⅱ对下丘脑促肾上腺皮质素释放素(CRH)和精氨酸加压素(AVP)分泌的作用.方法:低氧暴露采用人工模拟低压低氧.CRH和AVP的测定采用放射免疫测定法.血浆皮质酮测定采用荧光法.结果:7 km(8.2％O_2)急性低氧暴露1 h,AngⅡ不影响低氧引起的下丘脑CRH的分泌[低氧对照组:(15±4)ng/ME,低氧、脑室给Ang Ⅱ组:(160±8)ng/ME]但可增加下丘脑AVP的分泌[ME中AVP水平从低氧对照组(5.7±1.6)ng/ME下降到低氧、脑室给A Ⅱ组(2.6±1.2)ng/ME],同时血浆皮质酮水平也升高[从(356±58)μg/L plasma升高到(536±134)μg/L plasma],此作用可被AVP抗体部分反转.结论:急性低氧条件下,Ang Ⅱ对CRH的分泌无明显直接作用,它可能通过增加下丘脑AVP的分泌进而刺激下丘脑-垂体-肾上腺皮质轴的功能.
AIM: To investigate the effect of angiotensin Ⅱ on the secretion of corticotropin-releasing hormone (CRH) and arginine vasopressin (AVP) in hypothalamus under hypoxic conditions.Methods: Hypoxic exposure was performed by artificial simulated hypobaric hypoxia And AVP were measured by radioimmunoassay.The plasma corticosterone was measured by fluorescence method.Results: Ang Ⅱ did not affect the hypoxia-induced hypothalamic CRH secretion after exposure to acute hypoxia for 7 km (8.2% O 2) [Hypoxia control (15 ± 4) ng / ME, hypoxia, Ang Ⅱ group (160 ± 8) ng / ME] but increased the secretion of AVP in hypothalamus [AVP level in ME increased from 5.7 ± 1.6) ng / ME decreased to hypoxia and 2.6 ± 1.2 ng / ME in group A Ⅱ (mean ± SD), while the level of plasma corticosterone also increased (from 356 ± 58 μg / L plasma to 536 ± 134) μg / L plasma], and this effect can be partially reversed by the antibody of AVP.Conclusion: Under acute hypoxia conditions, Ang Ⅱ has no direct effect on the secretion of CRH, which may stimulate the hypothalamus by increasing the secretion of AVP in the hypothalamus - Pituitary-adrenal axis function.