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采用免疫组化LSAB法,用抗EGF-R单克隆抗体对38例喉鳞状细胞癌进行标记,用图像分析技术对染色结果定量分析,以阳性单位PU值代替染色级别(0~)。结果显示:喉鳞状细胞癌Ⅲ极的PU值明显高于Ⅰ~Ⅱ级的PU值,淋巴结转移组的PU值高于无淋巴结转移组的PU值,取得完整随访资料34例,绘制Kaplan-Meier生存曲线,经Logrank检验.X2=8.62,P<0.05。研究结果显示:EGF-R的表达状态与喉鳞癌的低分化性、高浸润性及预后密切相关,EGF-R可做为判断预后的一个良好的生物指标。
Thirty-eight laryngeal squamous cell carcinomas were labeled with anti-EGF-R monoclonal antibody using immunohistochemical LSAB method. The staining results were quantitatively analyzed by image analysis technique, and the positive unit PU value was used instead of the staining level (0 ~). The results showed that the PU value of laryngeal squamous cell carcinoma Ⅲ pole was significantly higher than that of Ⅰ ~ Ⅱlevel PU value, the PU value of lymph node metastasis group was higher than that of non-lymph node metastasis group, 34 cases were obtained, Meier survival curve, tested by Logrank. X2 = 8.62, P <0.05. The results show that the expression of EGF-R is closely related to the low differentiation, high invasiveness and prognosis of laryngeal squamous cell carcinoma. EGF-R can be used as a good biological index to judge the prognosis.