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目的：探讨不同种类的妇科肿瘤患者血清ＩＬ－６、ＴＮＦ－α和ＩＦＮ－α的表达。方法：采用双抗体夹心ＥＬＩＳＡ法和放射免疫法。结果：不同种类的妇科恶性肿瘤患者血清ＩＬ－６、ＴＮＦ－α和ＩＦＮ－α水平均明显高于不同种类的妇科良性肿瘤（Ｐ＜０．０１，Ｐ＜０．０５）。４种妇科恶性肿瘤血清ＩＬ－６的表达依次为：卵巢恶性肿瘤、恶性滋养叶细胞肿瘤、子宫内膜癌、宫颈癌，其均值分别为７８６．８９ｐｇ／ｍｌ、４１９．６７ｐｇ／ｍｌ、１８６．８０ｐｇ／ｍｌ、１４９．６７ｐｇ／ｍｌ。血清ＴＮＦ－α的表达依次为：宫颈癌、卵巢恶性肿瘤、恶性滋养叶细胞肿瘤、子宫内膜癌，其均值分别为８５０ｐｇ／ｍｌ，８４２．８６ｐｇ／ｍｌ、７２５ｐｇ／ｍｌ、７００ｐｇ／ｍｌ。血清ＩＦＮ－α的表达依次为：宫颈癌、卵巢恶性肿瘤、子宫内膜癌、恶性滋养叶细胞肿瘤。其均值分别为：２４３．３３ｐｇ／ｍｌ、２３７．８９ｐｇ／ｍｌ、２０１．４０ｐｇ／ｍｌ、１９８．７８ｐｇ／ｍｌ。结论：３种细胞因子的测定对恶性肿瘤的病情估计和预后判断有重要价值。
Objective: To investigate the expression of serum IL-6, TNF-α and IFN-α in different types of gynecological tumor patients. Methods: Double antibody sandwich ELISA and radioimmunoassay. Results: The levels of serum IL-6, TNF-α and IFN-α in patients with different types of gynecologic malignancy were significantly higher than those in different types of gynecological benign tumors (P<0.01, P<0.05). The expression of serum IL-6 in four types of gynecologic malignancies was ovarian cancer, malignant trophoblastic tumor, endometrial cancer, and cervical cancer. Their mean values were 786.89 pg/ml, 419.67 pg/ml, 186, respectively. 80 pg/ml, 149.67 pg/ml. The expression of serum TNF-α was: cervical cancer, ovarian cancer, malignant trophoblastic tumor, and endometrial cancer. The mean values of TNF-α were 850 pg/ml, 842.86 pg/ml, 725 pg/ml, and 700 pg/ml, respectively. The expression of serum IFN-α was as follows: cervical cancer, ovarian cancer, endometrial cancer, malignant trophoblastic tumor. The mean values were: 243.33 pg/ml, 237.89 pg/ml, 201.40 pg/ml, 198.78 pg/ml. Conclusion: The determination of three cytokines is of great value in the assessment of disease and prognosis of malignant tumors.