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为探讨胸腺哺育细胞(thymic nurse cell,TNC)与白血病发病的关系,我们用瑞氏染色和 ANAE、ACP、β-Gase 细胞化学方法,对接种白血病病毒 30d、75d 及正常组小鼠的外周血及 TNC 进行了观察比较。结果显示,30d 组外周血无明显改变,也未出现白血病发病征兆,但在 TNC 内可见体积较大的幼稚淋巴细胞,核仁大而清楚,与白血病细胞特征类似。TNC 胞质内三种细胞化学反应有局部性增强。TNC 内的淋巴细胞(TNC—L)ACP、β-Gase 阳性率显著增加;而 ANAE 则下降,结果显示幼稚淋巴细胞增加。75d 组,虽具有典型白血病体征,却很难分离到 TNC。本实验结果表明,在白血病发病过程中,TNC 首先出现改变。
To investigate the relationship between thymic nurse cells (TNC) and the pathogenesis of leukemia, WBC staining and ANAE, ACP, β-Gase cytochemical methods were used to study the relationship between leukemia virus-infected peripheral blood And TNC were observed and compared. The results showed no significant changes in the peripheral blood of the 30d group, and no signs of leukemia. However, in the TNC, larger lymphocytes were seen, with large and clear nucleoli, similar to those of leukemic cells. TNC cytoplasmic three kinds of cellular chemical reactions with local enhancement. The TNC-positive lymphocytes (TNC-L) ACP, β-Gase positive rate was significantly increased; ANAE decreased, the results showed that immature lymphocytes increased. 75d group, although with typical signs of leukemia, it is difficult to isolate TNC. The experimental results show that in the pathogenesis of leukemia, TNC first changed.