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为了对人类第9号染色体进行 SCE 分析,我们参照 Bobrow 等、Gagne 等的 Giemsa-11染色方法和 Alvec(3)的碱性溶液直接姊妹染色单体差别染色 Sister Chromatid Differentiation,SCD),摸索建立了同时显示 G—11染色和 SCD 的新方法,现介绍于下。一、细胞培养和染色体标本制备 经肝素抗凝的外周血0.3ml 接种于5ml 培养基内(含 RPMI16404ml、小牛血清1ml、广州产 PHA 400μg、青霉素、链霉素各500国际单位),置37℃培养24～26小时,加入BrdU(最终浓度10μg/ml)避光继续培养44～46小时,培养终止前2小时加入秋水仙素(最终浓度0.8
In order to perform SCE analysis on chromosome 9 of humans, we explored the method of Giemsa-11 staining by Bobrow et al., Gagne et al., and Sister Chromatid Differentiation (SCD) using Alvec’s (3) alkaline solution direct sister chromatid differential staining (SCD). A new method for simultaneously displaying G-11 staining and SCD is described below. I. Cell Culture and Preparation of Chromosomal Specimens 0.3 ml of heparin anticoagulated peripheral blood was inoculated into 5 ml of culture medium (containing RPMI 16404 ml, calf serum 1 ml, Guangzhou PHA 400 μg, penicillin, and streptomycin 500 units each), setting 37 Incubate for 24 to 26 hours at °C, add BrdU (final concentration 10 μg/ml) to protect from light for 44 to 46 hours, and add colchicine 2 hours before the termination of culture (final concentration 0.8