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[目的]观察不同亚型肠易激综合征(IBS)患者肠道菌群的改变,探讨IBS的发病机制。[方法]按照罗马Ⅲ标准选取腹泻型、便秘型及混合型IBS患者各20例,另选健康人20例作对照组,观察各组粪便菌群密集度、菌群多样性、细菌比例和菌群培养检测结果,并对比分析。[结果]腹泻组菌群密集度明显降低(P<0.05),且菌群多样性也明显低于对照组(P<0.05);与对照组比较,各亚型IBS患者革兰阳性杆菌菌群比例均明显下降(P<0.05)、革兰阴性杆菌比例明显升高(P<0.05),且腹泻组与便秘组、混合组之间也有显著差别(P<0.05);腹泻组、混合组革兰阳性球菌、大肠杆菌比例均较对照组有显著增高(P<0.05);腹泻组与便秘组比较亦有明显差别(P<0.05);混合组肠球菌与对照组比较差异有统计学意义(P<0.05);各亚型IBS患者双歧杆菌、乳杆菌均有减少,与对照组相比差异有统计学意义(P<0.05)。[结论]腹泻型、便秘型及混合型IBS患者均存在肠道菌群失调,以腹泻型最为明显,菌群失调应该是其发病的主要因素之一。
[Objective] To observe the changes of intestinal microflora in patients with different subtypes of irritable bowel syndrome (IBS) and to explore the pathogenesis of IBS. [Method] According to the standard of Rome Ⅲ, 20 cases of diarrhea, constipation and mixed IBS were selected and 20 healthy people were selected as control group. The concentration of fecal flora, flora diversity, bacterial ratio and bacteria Group culture test results, and comparative analysis. [Results] The concentration of bacteria in the diarrhea group was significantly lower (P <0.05), and the flora diversity was also significantly lower than that in the control group (P <0.05). Compared with the control group, the gram- (P <0.05). The proportion of Gram-negative bacilli was significantly increased (P <0.05), and there was a significant difference between diarrhea group and constipation group and mixed group (P <0.05). Diarrhea group, mixed group (P <0.05). There was also significant difference between diarrhea group and constipation group (P <0.05). There was significant difference between mixed group of Enterococci and control group (P <0.05) P <0.05). Bifidobacteria and lactobacilli in all subtypes of IBS patients decreased significantly compared with the control group (P <0.05). [Conclusion] The diarrhea type, constipation type and mixed type IBS patients have intestinal flora imbalance, diarrhea type is the most obvious, and flora should be one of the main factors.