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肿瘤宿主机体的免疫抑制状态与肿瘤来源的免疫抑制因子(TDISF)密切相关.本研究采用凝胶滤过层析和等电聚焦电泳技术,提取、纯化了人胃癌(SGC-7901)细胞TDISF,并探讨了其理化性状与免疫生物学特性.结果显示该肿瘤细胞TDISF的分子量为41KD、等电5.0,对强酸、强碱、加热(56℃)及胰蛋白酶均敏感,证实其化学性质为可溶性蛋白质类物质.该TDISF能明显地抑制T-细胞的增殖、抑制NK细胞和LAK细胞的杀伤活性,显示出剂量效应依赖关系.并对IL-2产生和活性及IL-2受体表达均有强烈的抑制作用,提示胃癌TDISF对T-细胞增殖、NK细胞和LAK细胞杀伤活性的抑制主要系通过抑制IL-2产生及活性所致.
The immunosuppressive state of the tumor host body is closely related to tumor-derived immunosuppressive factor (TDISF). In this study, gel filtration chromatography and isoelectric focusing electrophoresis were used to extract and purify the human gastric cancer (SGC-7901) cell TDISF. The physicochemical traits and immunobiological characteristics of the tumor cells were investigated. The results showed that the molecular weight of the tumor cell TDISF was 41KD, isoelectricity 5.0, and was sensitive to strong acids, strong bases, heating (56°C) and trypsin, confirming its chemical nature as soluble. Protein substances. The TDISF can significantly inhibit the proliferation of T-cells, inhibit the killing activity of NK cells and LAK cells, showing a dose-dependent dependence, and have both IL-2 production and activity and IL-2 receptor expression. The strong inhibitory effect suggested that the inhibition of gastric cancer TDISF on T-cell proliferation, NK cell and LAK cell killing activity is mainly caused by inhibition of IL-2 production and activity.