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目的研究黄芪对脑缺血再灌注大鼠脑神经细胞凋亡的影响,并通过对皮质及纹状体缺血周围区PI3-K和Akt的检测进一步探讨黄芪减少神经细胞凋亡的分子机制。方法采用ZeaLonga线栓法制备大鼠大脑中动脉闭塞(MCAO)模型,缺血3h后再灌注。分为假手术组、模型组、胞磷胆碱组、黄芪组。术后分12、24、48h3个时间点作神经功能评分;TUNEL法观察凋亡的情况;免疫组化法观察皮质及纹状体缺血周围区PI3-K和Akt表达强度。结果神经功能评分显示黄芪组和胞磷胆碱组恢复明显优于模型组,黄芪组和胞磷胆碱组相近;黄芪组凋亡阳性细胞数均少于模型组及胞磷胆碱组;模型组PI3-K和Akt免疫阳性细胞平均数显著低于黄芪组和胞磷胆碱组。于再灌注48h黄芪组与胞磷胆碱组间差异显著。结论黄芪可减轻大鼠脑缺血再灌注后神经细胞凋亡,能刺激PI3-K/Akt信号通路的表达。
Objective To study the effect of Astragalus membranaceus on apoptosis of cerebral neurons in rats with cerebral ischemia and reperfusion, and further explore the molecular mechanism of Astragalus membranaceus in reducing neuronal apoptosis through the detection of PI3-K and Akt in the cortex and striatum surrounding ischemic region. Methods The rat model of middle cerebral artery occlusion (MCAO) was prepared with Zea Longa thread embolism and reperfusion after 3h ischemia. Divided into sham operation group, model group, citicoline group, Huangqi group. The neurological scores were scored at 12, 24, and 48 hours after surgery. Apoptosis was observed by TUNEL. Immunohistochemistry was performed to examine the expression of PI3-K and Akt in the cortex and striatum. Results The neurological function scores showed that the recovery of the Astragalus group and citicoline group was significantly better than that of the model group. The Astragalus group and the citicoline group were similar; the number of apoptotic positive cells in the Astragalus group was lower than that in the model group and the citicoline group; The average number of PI3-K and Akt immunoreactive cells in the group was significantly lower than that in the Astragalus group and the citicoline group. There were significant differences between the jaundice group and citicoline group at 48h after reperfusion. Conclusion Astragalus membranaceus can reduce neuronal apoptosis after cerebral ischemia-reperfusion in rats and stimulate the expression of PI3-K/Akt signaling pathway.