论文部分内容阅读
目的建立连花清瘟胶囊水提工艺中间体快速含量测定方法。方法采用UPLC测定新绿原酸、绿原酸、甘草苷、3,4-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸、甘草酸的含量。色谱柱为ACQUITY UPLC-BEH C18色谱柱(2.1 mm×100 mm,1.7μm);流动相为甲醇-乙腈-0.1%磷酸;检测波长:237 nm。结果新绿原酸在4.32~21.6 ng内线性良好(r=0.999 8)、绿原酸在6.82~34.08 ng内线性良好(r=0.999 8)、甘草苷在2.49~9.33 ng内线性良好(r=0.999 8)、3,4-二咖啡酰奎宁酸在16.35~81.76 ng内线性良好(r=0.999 7)、4,5-二咖啡酰奎宁酸在14.42~72.08 ng内线性良好(r=0.999 7)、甘草酸在4.19~20.96 ng内线性良好(r=0.999 6),平均回收率(n=6)分别为97.10%,96.66%,97.34%,98.98%,98.97%,97.80%,RSD分别为0.82%,1.30%,1.31%,1.17%,1.63%,1.73%。结论本方法简便、快速、准确,可有效地控制连花清瘟胶囊水提液的质量。
OBJECTIVE To establish a rapid method for the determination of the rapid substance in the process of water extraction of Lianhua Qingwen capsule. Methods UPLC was used to determine the content of chlorogenic acid, chlorogenic acid, glycyrrhizin, 3,4-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid and glycyrrhizic acid. The column was ACQUITY UPLC-BEH C18 (2.1 mm × 100 mm, 1.7 μm). The mobile phase was methanol-acetonitrile-0.1% phosphoric acid. The detection wavelength was 237 nm. Results The linearity was good (r = 0.999 8) for chlorogenic acid between 4.32 and 21.6 ng, linear between 6.82 and 34.08 ng for chlorogenic acid (r = 0.999 8), and between 2.49 and 9.33 ng for glycyrrhizin (r = 0.999 8). The linearity of 3,4-dou caffeoylquinic acid in the range of 16.35 ~ 81.76 ng (r = 0.999 7) and the linearity of 4,5-dou caffeoylquinic acid in the range of 14.42 ~ 72.08 ng (r = 0.999 7). The average recoveries of glycyrrhizin in the range of 4.19 ~ 20.96 ng were 97.10%, 96.66%, 97.34%, 98.98%, 98.97%, 97.80%, respectively Respectively 0.82%, 1.30%, 1.31%, 1.17%, 1.63%, 1.73%. Conclusion The method is simple, rapid and accurate, which can effectively control the quality of Lianhua Qingwen capsule water extract.