论文部分内容阅读
[目的]研究辛伐他汀对体外培养的成人骨髓基质细胞成骨分化和成脂分化的影响,并探讨其作用机制。[方法]分离健康成人骨髓基质细胞进行培养,传代后骨分化诱导组和脂肪分化诱导组分别添加10-7mol/L的辛伐他汀,同时进行空白对照。实时荧光定量PCR检测转录因子Cbfa1在成骨细胞中的分化,PPARγ2和LPL在脂肪细胞分化过程中的表达;碱性磷酸酶(ALP)检测试剂盒检测成骨细胞ALP比活性;流式细胞仪、油红O(oilredO)染色检测细胞成脂分化能力。[结果]骨分化诱导组Cbfa1表达(P﹤0.01)、ALP比活性(P﹤0.05)均高于对照组,脂肪分化诱导组PPARγ2(P﹤0.01)、LPL(P﹤0.05)表达均低于对照组;流式细胞仪脂肪细胞计数及脂肪细胞形成脂滴能力实验组均低于对照组。[结论]10-7mol/L辛伐他汀能促进Cbfa1的表达,增强成骨细胞ALP比活性和细胞外基质矿化;抑制PPARγ2、LPL的表达及脂肪细胞分化。
[Objective] To study the effect of simvastatin on osteogenic differentiation and adipogenic differentiation of adult bone marrow stromal cells cultured in vitro and its mechanism of action. [Methods] Bone marrow stromal cells of healthy adults were isolated and cultured. After passage, osteogenic differentiation group and adipogenic differentiation group were treated with 10-7mol / L simvastatin respectively, and blank control was also performed. Real-time fluorescent quantitative PCR was used to detect the differentiation of transcription factor Cbfa1 in osteoblasts and the expression of PPARγ2 and LPL during adipocyte differentiation. ALP assay kit was used to detect the specific activity of ALP in osteoblasts. Flow cytometry , Oil red O staining to detect adipogenic differentiation ability of cells. [Results] The expression of Cbfa1 in osteogenic differentiation group (P <0.01) and ALP specific activity (P <0.05) were higher than those in control group. The expression of PPARγ2 in adipogenic differentiation group (P <0.01) and LPL The control group, the flow cytometry adipocyte count and adipocytes to form lipid droplets ability of the experimental group were lower than the control group. [Conclusion] 10-7mol / L simvastatin can promote the expression of Cbfa1, enhance the specific ALP activity and extracellular matrix mineralization of osteoblasts, inhibit the expression of PPARγ2, LPL and adipocyte differentiation.