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[目的]研究濒危植物香果树愈伤组织经长期继代后的6种同工酶酶谱的变化。[方法]采用非变性聚丙烯凝胶电泳技术对长期继代后的香果树愈伤组织的酯酶(EST)、酸性磷酸酯酶(ACP)、ATP酶(ATPase)、淀粉酶(AMY)、超氧化物歧化酶(SOD)和过氧化物酶(POD)6种同工酶进行分析。[结果]香果树的胚性愈伤组织和非胚性愈伤组织在6种同工酶水平上均有差异,均可作为鉴别2者的依据。非胚性愈伤组织中的EST、ACP和POD的表达量明显高于胚性愈伤组织。褐化的非胚性愈伤组织与正常非胚性愈伤组织相比,在AMY、SOD和POD同工酶水平上无差别,而EST、ACP和ATPase同工酶含量减少;褐化的胚性愈伤组织与正常胚性愈伤组织相比,EST同工酶含量升高,其他5种同工酶含量降低。[结论]为研究香果树的长期组织培养过程中愈伤组织形态差异及褐化现象提供理论基础。
[Objective] The study aimed to study the changes of 6 isozymes of callus from the endangered plant Emmenopterys henryi by long-term subculture. [Method] The ESTs, ACP, AMY, AMY and AMY of callus were studied by non-denaturing polyacrylamide gel electrophoresis (PAGE) Superoxide dismutase (SOD) and peroxidase (POD) 6 isozymes were analyzed. [Result] The embryogenic callus and non - embryogenic callus of the fragrant fruit tree all had the difference in 6 kinds of isozymes, can be used as the basis of distinguishing 2 people. The expression of EST, ACP and POD in non-embryogenic callus was significantly higher than that in embryogenic callus. Compared with normal non-embryogenic calli, browning non-embryogenic callus showed no difference in AMY, SOD and POD isozymes but decreased EST, ACP and ATPase isozymes. Browning embryos Compared with normal embryogenic calli, the content of EST isoenzyme increased and the content of other five isoenzymes decreased. [Conclusion] The study provided the theoretical basis for studying the morphological differences and browning of calli during long-term tissue culture.