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从炼油厂污泥中分离出一株能专一脱除二苯并噻吩(dibenzothiophene,DBT)中的硫,并生成2 羟基联苯(2 hy droxybiphenyl,2 HBP)的棒杆菌属(Corynebacterium)菌株ZD 1.ZD 1能够利用DBT作为惟一硫源,以甘油作为碳源,用摇瓶培养法测定了该菌株的生长和脱硫曲线.该菌株在培养45h时菌体质量浓度达到最大,在指数生长后期的菌体脱硫活性最高,2 HBP的比生成速率可达0.14(mmol/(kg·min)).同时还考察了影响该菌株生长的初始pH、碳源类型及其质量浓度、硫源类型及其浓度以及氮源质量浓度和生长温度.ZD 1能利用多种碳源和硫源,其中以甘油和DBT为最适宜.结果表明,pH=6.0～7.5,10g/L的甘油,0.2mmol/L的DBT,5g/L的氯化铵,温度为30℃时ZD 1的生长最好.
A strain of Corynebacterium that specifically removes sulfur from dibenzothiophene (DBT) and produces 2-hy droxybiphenyl (2 HBP) was isolated from the refinery sludge ZD 1.ZD 1 can use DBT as the only sulfur source, glycerol as carbon source, shake flask culture was measured the strain growth and desulfurization curve of the strain at 45h cultured bacteria mass concentration maximum, exponential growth At the same time, the initial pH value, carbon source type and mass concentration, the type of sulfur source And its concentration as well as the concentration of nitrogen source and growth temperature.ZD 1 can use a variety of carbon sources and sulfur sources, of which glycerol and DBT are the most suitable.The results show that the pH = 6.0 ~ 7.5,10 g / L of glycerol, 0.2mmol / L of DBT, 5g / L of ammonium chloride, the temperature of 30 ℃ ZD 1 the best growth.