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为探讨链脲菌素脑室内注射大鼠建立的Ａｌｚｈｅｉｍｅｒ病模型脑组织的损伤机制和乙酰肉毒碱的神经保护作用，测定脑中隔和海马回α－酮戊二酸脱氢酶（α－ＫＧＤＨ）、谷氨酸脱氢酶（ＧＬＤＨ）、胆碱乙酰基转移酶（ＣｈＡＴ）、肉毒碱乙酰基转移酶（ＣＡＴ）的酶活性。结果显示，脑中隔α－ＫＧＤＨ和ＧＬＤＨ活性均下降，并且酶活性的降低与中隔重量的减轻具有高度相关性。乙酰肉毒碱能提高海马回被链脲菌素降低的ＣｈＡＴ的活性。提示链脲菌素是通过对脑组织的损伤作用影响酶活性的；乙酰肉毒碱对脑组织具有神经保护作用。
To investigate the damage mechanism of brain tissue of Alzheimer’s disease model induced by intracerebroventricular injection of streptozotocin and the neuroprotective effect of acetyl-carnitine, the levels of α-ketoglutarate dehydrogenase (α- KGDH), glutamate dehydrogenase (GLDH), choline acetyltransferase (ChAT) and carnitine acetyltransferase (CAT). The results showed that the activity of α-KGDH and GLDH in septum decreased, and the decrease of enzyme activity was highly correlated with the reduction of septum weight. Acetyl-carnitine increases the activity of ChAT which is reduced by streptozotocin in the hippocampus. It suggests that streptozotocin affects the activity of the enzyme through the damage of brain tissue. Acetylglucosamine has neuroprotective effect on brain tissue.