采用四甲基偶氮唑蓝 ( MTT)法进行检测 ,观察 32例肺癌恶性胸水标本对 6种常用抗癌药物在 5倍 ,1倍和 0 .2倍血浆峰值浓度时的抑制作用。结果 :在药物血浆峰值浓度 1倍时足叶乙甙、顺铂、阿霉素、丝裂霉素、长春新碱与氨甲喋呤的敏感率分别为 84.3%、65 .6%、40 .6%、 2 8.1 %、1 8.7%、1 2 .5 % ;而在 5倍血浆峰值浓度时敏感率分别为 90 .6%、 81 .3%、 68.7%、5 3.1 %、43.7%、2 1 .8% ;在 0 .2倍血浆峰值浓度时敏感率分别为 1 5 .6%、1 2 .5 %、0 .6%、0、0、0。提示 :肺癌患者对化疗药物的敏感性存在较大的个体差异 ,而采用浓度梯度法药敏实验 ,对于避免在化疗中盲目用药 ,提高化疗效果 ,具有重要的临床指导意义 The MTT assay was used to detect the inhibitory effect of 32 samples of malignant pleural effusion on the 6 commonly used anticancer drugs at 5 times, 1 times and 0.2 times of peak plasma concentration. Results: The sensitivities of etoposide, cisplatin, doxorubicin, mitomycin, vincristine and methotrexate were 84.3%, 65.6% and 40.6% at the peak plasma concentration of 1, 2 8.1%, 18.7% and 12.5%, respectively; while at 5 times the plasma peak concentration, the sensitivity rates were 90.6%, 81.3%, 68.7%, 51.1%, 43.7%, 21.8% %; At 0.2 times the plasma peak concentration, the sensitivity rates were 15.6%, 12.5%, 0.6%, 0,0,0 respectively. Tip: there is a large individual difference in chemosensitivity of lung cancer patients, and the use of concentration gradient method susceptibility testing for the avoidance of chemotherapy in the blind to improve the efficacy of chemotherapy has important clinical significance