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目的通过检测结核性和癌性胸腔积液中IL-16水平,探讨其与T淋巴细胞亚群之间的关系。方法收集62例胸腔积液(32例结核性和30例癌性)患者的胸腔积液与静脉血,ELISA测胸腔积液上清液及血清中IL-16水平;流式细胞仪测胸腔积液中T淋巴细胞亚群;行胸腔积液中细胞计数及分类。结果62例胸腔积液者,胸腔积液中IL-16水平高于其血清水平。结核性胸腔积液中IL-16水平高于癌性胸腔积液中IL-16水平。胸腔积液中IL-16水平与淋巴细胞总数、T细胞数及CD4+细胞数呈正相关(r分别为0.526、0.638、0.701,P值均小于0.001)。结论与癌性胸腔积液相比,结核性胸腔积液中IL-16水平高,IL-16有可能在CD4+T细胞浸润到胸腔的过程中具有重要的趋化作用。
Objective To investigate the relationship between T-lymphocyte subsets and IL-16 levels in tuberculous and cancerous pleural effusions. Methods Pleural effusion and venous blood samples were collected from 62 cases of pleural effusion (32 cases of tuberculous and 30 cases of cancerous), IL-16 levels in serum and pleural effusion by ELISA and flow cytometry Fluid T lymphocyte subsets; pleural effusion cell count and classification. Results 62 cases of pleural effusion, pleural effusion IL-16 levels higher than its serum level. IL-16 levels in tuberculous pleural effusion are higher than those in cancerous pleural effusions. The level of IL-16 in pleural effusion was positively correlated with the total number of lymphocytes, T cells and CD4 + cells (r = 0.526,0.638,0.701, P <0.001). Conclusion Compared with cancerous pleural effusion, IL-16 is highly expressed in tuberculous pleural effusion, and IL-16 may play an important chemotactic role in infiltration of CD4 + T cells into pleural cavity.