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本文提出了一种单向一步SDS-PAGE(聚丙烯酰胺凝胶电泳)分析小麦贮藏蛋白(谷蛋白和醇溶蛋白)的方法,利用SDS-PAGE重新评价普通小麦品种间染色体代换系醇溶谷蛋白的组成.从小麦品种Cheyenne和Wichita的第1和第6组染色体的轮回代换系中提取谷蛋白和醇溶蛋白,估计每个品种特有蛋白质的分子量,第1组染色件编码的蛋白质,特别是醇溶蛋白和低分子谷蛋白亚基比第6组染色体编码的蛋白质变异大,高分子和低分子谷蛋白亚基都微溶于乙醇溶液.和四交品种相比,Cheyenne和Wichita的染色体代换系的醇溶谷蛋白多肽链都产生了变化.因而,只有在分析了两种醇溶谷蛋白组分后,才能确定品质改变(和代换染色体有关)和贮藏蛋白之间的相关性.
In this paper, we present a method for SDS-PAGE (polyacrylamide gel electrophoresis) analysis of wheat storage proteins (gluten and prolamin) using SDS-PAGE to re-evaluate the alcohol- Gluten composition Gluten and prolamin were extracted from the reciprocal lines of the first and sixth chromosomes of the Cheyenne and Wichita wheat cultivars to estimate the molecular weight of each of the cultivars specific to the protein encoded by the first group of stains Especially gliadin and low-molecular-weight glutenin subunits were larger than those encoded by chromosome 6, and both macromolecules and low-molecular-weight glutenin subunits were slightly soluble in ethanol.Compared with the four crosses, Cheyenne and Wichita Of the chromosome substitution lines have changed in the prolamin polypeptide chain.Thus, only after analyzing two prolamin components can one determine the difference between the quality change (and the substitution chromosome) and the storage protein Correlation.