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本研究对基因工程人白介素-6成熟肽在大肠杆菌中的表达、纯化及检定做了一系列的研究,建立了一套罐培养诱导表达,乙酸裂解包涵体,复性及纯化的中试工艺,并制备3批半成品,经检定各项指标已接近国家基因工程产品质控标准。
In this study, a series of researches on the expression, purification and identification of genetically engineered human interleukin-6 mature peptide in Escherichia coli were carried out. A series of pilot studies were conducted to establish a can-culture-induced expression, acetic acid lytic inclusion body, renaturation and purification , And prepared three batches of semi-finished products, the test indicators have been close to the national quality standards for genetic engineering products.