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利用感染人类幽门螺杆菌（H．pyloi）的小鼠模型，研究H．pylori粗制抗原与霍乱毒素（CT）或重组霍乱毒素B亚单位（rCTB）组成的口服疫苗预防H．pylori感染的作用。方法：无特殊病原菌级C57BL／6小鼠分为4组，通过灌胃方法分别予各组小鼠以H．pylori超声粉碎抗原1mg加CT10μg、H．pylori超声粉碎抗原1mg加rCTB10μg、单纯H.pylori超声粉碎抗原1mg和生理盐水，每周1次，共4次。最后1次免疫后1周以5×108／只的活力悉尼株H．pylori攻击小鼠，隔日1次，共3次。最后1次攻击后2周处死小鼠，取胃粘膜分别行快速尿素酶试验、涂片、培养及组织学检查，鉴定H．pylori感染状况。结果：H．pylori超声粉碎抗原加CT组对H.pylori感染的保护率为4／6，H．pylori超声粉碎抗原加rCTB组与单纯H.pylori超声粉碎抗原组的保护率为1／7，生理盐水组的保护率为0／6。结论：H．pylori超声粉碎抗原加CT组成的日服疫苗具有预防H．pylori感染的作用，rCTB的佐剂作用不明显。
H. Using a mouse model infected with H. pylori (H. Oral vaccine prophylaxis consisting of crude pylori antigen and cholera toxin (CT) or recombinant cholera toxin B subunit (rCTB). The role of pylori infection. Methods: C57BL / 6 mice without special pathogens were divided into 4 groups, and mice in each group were given H. By gavage respectively. pylori ultrasound crushed antigen 1mg plus CT10μg, H. pylori ultrasonic crushing antigen 1mg plus rCTB10μg, pure H. pulvis pulsed ultrasound antigen 1mg and saline once a week for a total of 4 times. One week after the last immunization, 5 × 108 per mouse of viable Sydney strain H was used. Pylori challenge mice, every other day, a total of 3 times. Mice were sacrificed 2 weeks after the last attack, and the gastric mucosa was taken for rapid urease test, smear, culture and histological examination, respectively. pylori infection status. Results: H The protection rate of H.pylori infection by pylori sonicated antigen and CT group was 4/6, H. The protection rate of pylori ultrasonic milled antigen plus rCTB group and pure H.pylori ultrasonic milled antigen group was 1/7, and that of saline group was 0/6. Conclusion: H H. pylori sonicated antigen combined with CT composition of the Japanese-made vaccine has preventive H. The role of pylori infection, rCTB adjuvant effect is not obvious.