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目的:建立中药艾复康胶囊中黄芩苷含量的测定方法。方法:采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),0.4%磷酸-甲醇(58∶42)为流动相,检测波长280 nm,流速1.0 mL.min-1,柱温35℃。结果:黄芩苷线性方程为Y=2.78×103X-4.13,在0.096 8-0.484μg范围内具良好的线性关系(r=0.999 7),平均回收率为99.7%,RSD为3.0%。结论:该法准确、可靠、重复性好,可用于艾复康胶囊中黄芩苷含量的测定。
Objective: To establish a method for the determination of baicalin in Aifukang capsules. Methods: Diamonsil C18 column (250 mm × 4.6 mm, 5 μm), 0.4% phosphoric acid-methanol (58:42) as mobile phase, detection wavelength 280 nm, flow rate 1.0 mL.min-1, column temperature 35°C. RESULTS: The linear equation of baicalin was Y=2.78×103X-4.13, with a good linearity in the range of 0.096 8-0.484 μg (r=0.999 7). The average recovery was 99.7% and the RSD was 3.0%. Conclusion: The method is accurate, reliable and reproducible. It can be used for the determination of baicalin in Ai Fukang capsules.