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目的:研究芹菜素(API)是否具有敏化肿瘤坏死因子相关凋亡诱导配体(TRAIL)诱导人卵巢癌CoC1细胞凋亡作用。方法:体外培养CoC1细胞。碘化丙啶(PI)染色流式细胞术(FCM)定量分析sub-G1细胞百分率。ELISA法测定细胞Caspase-3活性。结果:API(20μmol/L)和TRAIL(20ng/mL)以及两者合用48h的sub-G1细胞百分率分别是8.83%±2.33%、8.32%±2.80%和69.50%±4.65%;人卵巢癌CoC1细胞Caspase-3的活性分别是培养基组的1.3、1.4和6.5倍。结论:亚细胞毒性浓度的芹菜素具有增强TRAIL诱导人卵巢癌CoC1细胞凋亡作用。
AIM: To investigate whether apigenin (API) can sensitize apoptosis induced by TRAIL to human ovarian cancer CoC1 cells. Methods: CoC1 cells were cultured in vitro. Propidium iodide (PI) staining flow cytometry (FCM) Quantitative analysis of sub-G1 cell percentage. The activity of Caspase-3 was determined by ELISA. Results: The percentage of sub-G1 cells treated with API (20μmol / L) and TRAIL (20ng / mL) for 48h was 8.83% ± 2.33%, 8.32% ± 2.80% and 69.50% ± 4.65% Cell Caspase-3 activity was 1.3, 1.4 and 6.5 fold, respectively, in the media group. CONCLUSION: Apigenin with sub-cytotoxic concentration can enhance the apoptosis induced by TRAIL in human ovarian cancer CoC1 cells.