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目的探讨中药珍珠梅黄酮纳米粒(5,2’,4’-trihydroxy-6,7,5’-trimethoxy flavone nanoparticle,TTF1-NP)调控STAT3抑制人肝癌Hep G2细胞侵袭、转移的作用和分子机制。方法利用Western blot技术检测人肝癌HepG2细胞STAT3和p-STAT3蛋白表达,利用Transwell和细胞划痕技术检测人肝癌Hep G2细胞侵袭和转移能力,采用Western blot技术检测细胞侵袭迁移的相关蛋白MMP2和MMP9表达。结果 Western blot检测发现TTF1-NP可以抑制人肝癌Hep G2细胞STAT3的活化表达,通过抑制MMP2和MMP9表达抑制人肝癌Hep G2细胞侵袭和转移。结论 TTF1-NP通过抑制STAT3的活化抑制MMP2和MMP9蛋白表达抑制人肝癌Hep G2细胞侵袭和转移。
Objective To investigate the effect and molecular mechanism of STAT3 on the invasion and metastasis of human hepatocellular carcinoma Hep G2 cells by 5,2 ’, 4’-trihydroxy-6,7,5’-trimethoxythione flavone nanoparticle (TTF1-NP) . Methods Western blot was used to detect the expression of STAT3 and p-STAT3 protein in HepG2 cells. The invasion and metastasis of Hep G2 cells were detected by Transwell and cell scratch assay. The expressions of MMP2 and MMP9 were detected by Western blot expression. Results Western blot showed that TTF1-NP could inhibit the activation of STAT3 in Hep G2 cells and inhibit the invasion and metastasis of Hep G2 cells by inhibiting the expression of MMP2 and MMP9. Conclusion TTF1-NP can inhibit the invasion and metastasis of Hep G2 cells by inhibiting the activation of STAT3 and inhibiting the expression of MMP2 and MMP9.