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[Objective]The aim was to study the preparation of salidroside basing on modern biotechnology. [Method] Basing on SDS-PAGE method, the expression condition of the recombinant gene was optimized by investigating the effects of induction temperature, concentration of IPTG and induction time on the gene expression. [Result]The optimum induction conditions were as follows: induction time of 5 h, IPTG concentration of 0.6 mmol/L and induction temperature of 37 ℃. The target protein with purity of 99% was obtained by purifying the fusion protein with Ni-chromatography column. [Conclusion]The study will provide successful experience for the purification of tyrosol glycosyltransferase.
[Objective] The aim was to study the preparation of salidroside basing on modern biotechnology. [Method] Basing on SDS-PAGE method, the expression condition of the recombinant gene was optimized by investigating the effects of induction temperature, concentration of IPTG and induction time on the gene expression. [Result] The optimum induction conditions were as follows: induction time of 5 h, IPTG concentration of 0.6 mmol / L and induction temperature of 37 ° C. The target protein with purity of 99% was obtained by purifying the fusion protein with Ni-chromatography column. [Conclusion] The study will provide successful experience for the purification of tyrosol glycosyltransferase.