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为了探讨肝炎后肝硬化病人外周血T淋巴细胞亚群及其核仁形成区嗜银蛋白(AgNORs)酸性非组蛋白表达活性的变化与临床意义,我们运用KL型计算机免疫图像分析系统及碱性磷酸酶抗碱性磷酸酶法(APAAP法)对75例肝炎后肝硬化患者及30例正常人外周血T淋巴细胞亚群及其AgNORs进行检测,报告如下。1对象及方法1.1对象75例肝炎后肝硬化病人为我院2002年8-11月住院病人,诊断标准参照2000年全国第十次(西安)传染病与寄生虫病会议修订的诊断标准,男52例,女23例,年龄31-62岁,HbsAg(+)者65例,抗HCV(+)者8例,二者同时阳性者2例,临床上排除遗传性疾病及其他系统疾病。正常对照组为门诊体检献血员30例。1.2方法(1)AgNORs活性测定:无菌条件下取0.5ml抗
In order to explore the changes and clinical significance of T lymphocyte subsets and the expression of AgNORs in patients with posthepatitic cirrhosis, we used KL type computerized immune image analysis system and alkaline Phosphatase anti-alkaline phosphatase (APAAP method) on 75 cases of hepatitis and cirrhosis patients and 30 cases of normal peripheral blood T lymphocyte subsets and AgNORs were detected, the report is as follows. 1 Subjects and methods 1.1 Object 75 cases of hepatitis cirrhosis patients for our hospital from August to November 2002 inpatients, the diagnostic criteria with reference to the 2000 national tenth (Xi’an) Infectious Diseases and Parasitic Diseases meeting revised diagnostic criteria, male 52 cases and 23 females, aged 31-62 years old, 65 cases of HbsAg (+), 8 cases of anti-HCV (+), both were positive at the same time in 2 cases, clinically excluded genetic diseases and other systemic diseases. The normal control group was outpatient examination blood donors 30 cases. 1.2 Methods (1) AgNORs activity determination: Take 0.5ml anti-aseptic conditions