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为构建河南旱稻种植品种的DNA指纹图谱,了解河南旱稻的遗传多样性,本研究利用均匀分布于水稻12条染色体的38对SSR引物,以河南主要推广种植的10个旱稻品种为材料,进行遗传多样性分析并构建SSR指纹图谱。结果表明,35对引物具有多态性片段,共检测到108个等位基因,平均每位点3.1个等位基因;引物平均多态性频率为0.485。10个品种间的遗传相似系数为0.435~0.907,平均0.678。利用RM552、RM171、RM5、RM308、RM338、RM17和RM587 7对多态性较高的SSR引物构建的核心指纹图谱能区分出10个旱稻品种。采用非加权类平均法进行聚类分析,在遗传相似系数0.480处可将10个品种分为籼、粳两大类。
In order to understand the genetic diversity of upland rice in Henan province, 38 pairs of SSR primers uniformly distributed on 12 chromosomes of rice were used to construct 10 DNAs of upland rice cultivated in Henan Province. Diversity analysis and construction of SSR fingerprinting. The results showed that 35 pairs of primers had polymorphic fragments, a total of 108 alleles were detected with an average of 3.1 alleles per locus; the average polymorphism frequency of primers was 0.485. The genetic similarity coefficient between the 10 cultivars was 0.435 ~ 0.907, averaging 0.678. The core fingerprinting of RM552, RM171, RM5, RM308, RM338, RM17 and RM587 7 can distinguish 10 Upland rice varieties from SSR primers with high polymorphism. Cluster analysis using unweighted average method, the genetic similarity coefficient of 0.480 can be divided into 10 varieties indica, Japonica two categories.