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为了研究基因诊断在皮肤 T 细胞淋巴瘤(CTCL)的临床应用,采用聚合酶链反应(PCR)技术,对60例皮肤淋巴细胞浸润疾患进行了 T 细胞受体(TCR)基因重排的检测。结果显示,检出TCR-β和(或)TCR-γ基因克隆重排的有:36/40例 CTCL、4/6例可疑蕈样肉芽肿/Sézary 综合征和1/1例淋巴瘤样丘疹病;未检出 TCR-β或γ基因克隆重排的有:2例皮肤 B 细胞淋巴瘤、1例皮肤淋巴细胞瘤、8例良性皮肤淋巴细胞浸润疾患和2例正常人皮肤。这表明:该方法在确定 CTCL 的克隆性、细胞源性以及早期诊断方面是有帮助的。
In order to study the clinical application of gene diagnosis in cutaneous T-cell lymphoma (CTCL), polymerase chain reaction (PCR) technique was used to detect the T cell receptor (TCR) gene rearrangement in 60 cases of skin lymphocytic infiltration disorders. The results showed that 36/40 CTCL, 4/6 suspected mycosis/Sézary syndrome, and 1/1 lymphoma-like papule were detected in the TCR-β and/or TCR-γ gene clone rearrangements. Disease; TCR-β or γ gene clone rearrangements were not detected: 2 cases of cutaneous B-cell lymphoma, 1 case of cutaneous lymphoma, 8 cases of benign dermal lymphocyte infiltration disease and 2 cases of normal human skin. This indicates that this method is helpful in determining the clonality, cell-derived and early diagnosis of CTCL.