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采用高效液相色谱法对甘蓝内叶、外叶、漂烫及真空干燥后的硫苷含量进行了成分鉴定及组分分析。结果表明,甘蓝内叶与外叶的组分相同,通过HPLC-ESI-MS共鉴定出5种脂肪族硫苷(3-甲基硫氧丙基GS,2-羟基-3-丁烯基GS,2-丙烯基GS,3-丁烯基GS,4-戊烯基GS)和1种吲哚族硫苷(3-吲哚基甲基GS),甘蓝中主要硫苷成分为2-羟基-3-丁烯基GS,2-丙烯基GS,3-吲哚基甲基GS及3-甲基硫氧丙基GS,内叶和外叶中硫苷含量分别为1972μg/g干重和1941μg/g干重,漂烫和真空干燥后硫苷的保存率分别为87.7%和37.4%。
High performance liquid chromatography (HPLC) was used to identify and analyze the content of glucosinolate in cabbage leaves, outer leaves, blanching and vacuum drying. The results showed that the components of the inner and outer leaves of cabbage were the same. Five aliphatic glucosinolates (3-methylthioxypropyl GS, 2-hydroxy-3-butenyl GS , 2-propenyl GS, 3-butenyl GS, 4-pentenyl GS) and one indole glucosinolate (3-indolylmethyl GS) Butenyl GS, 2-propenyl GS, 3-indolylmethyl GS and 3-methylthioxypropyl GS, the contents of glucosinolates in the inner and outer leaves were respectively 1972 μg / g dry weight and 1941μg / g dry weight, blanching and vacuum drying after the preservation rate of glucosinolate were 87.7% and 37.4%.