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对不同产地麻疯树脱油种仁中的总生物碱进行提取,采用酸性染料比色法,以盐酸川芎嗪为对照品,于420 nm处测定总生物碱含量,并利用单因素法考察最佳提取条件.结果表明,盐酸川芎嗪在0.036～0.180 mg mL-1范围内线性关系良好(R2=0.999 9),平均加样回收率为98.19%,RSD为2.53%(N=6).7个不同产地麻疯树脱油种仁中总生物碱含量在3.817～4.467 mg g-1之间,其中四川攀枝花地区的总生物碱含量最高.该方法操作简便,准确可靠,重复性好,可作为麻疯树脱油种仁总生物碱的含量测定方法.
Total alkaloids extracted from different kinds of Jatropha curcas dehumidification seeds were extracted by Acid Dye Colorimetry and Tetramethylpyrazine Hydrochloride was used as reference substance to determine the total alkaloid content at 420 nm. The results showed that ligustrazine hydrochloride had good linearity (R2 = 0.999 9) in the range of 0.036 ~ 0.180 mg mL-1 with an average recovery of 98.19% and a RSD of 2.53% (N = 6) .7 The total alkaloid content of Jatropha curcas dehumidification seeds in different habitats ranged from 3.817 to 4.467 mg g-1, of which the total alkaloid content was the highest in Panzhihua, Sichuan Province.The method was simple, accurate, reliable and reproducible As Jatropha tree oil total alkaloids content determination method.