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为探讨ＩＬ－６在狼疮性肾炎病人肾脏组织中的表达情况及其致病作用，我们用ＲＴ－ＰＣＲ和原位杂交方法对狼疮性肾炎病人和正常人肾穿标本中的ＩＬ－６ｍＲＮＡ进行检测。结果：（１）用ＲＴ－ＰＣＲ方法检测的５例病人都有ＩＬ－６ｍＲＮＡ的表达，而３例正常对照中仅一例有弱的ＩＬ－６ｍＲＮＡ的表达。（２）用地高辛标记的ＩＬ－６ｃＤＮＡ探针，对１３例病人进行原位杂交检测，结果都有不同程度ＩＬ－６ｍＲＮＡ的表达，表达部位在肾小球系膜细胞、小管上皮细胞、血管内皮细胞和间质细胞等。而３例正常对照未检测到ＩＬ－６ｍＲＮＡ的表达。表明在狼疮性肾炎病人肾脏组织中ＩＬ－６ｍＲＮＡ表达增高，且ＩＬ－６ｍＲＮＡ的高表达与血清中ＡＮＡ水平及肾脏ＩｇＧ、Ｃ３沉积可能有关。提示ＩＬ－６在狼疮性肾炎发病中可能有重要的病理作用。
To investigate the expression and pathogenicity of IL-6 in renal tissue of patients with lupus nephritis, we detected the IL-6 mRNA in renal peritoneitis patients and normal human by using RT-PCR and in situ hybridization . Results: (1) The expression of IL-6 mRNA was detected in all the 5 patients detected by RT-PCR, while only one of the 3 normal controls had weak IL-6 mRNA expression. (2) Using digoxigenin labeled IL-6cDNA probe, 13 patients were detected by in situ hybridization, the results have different levels of IL-6mRNA expression, the expression site in the mesangial cells, tubular epithelial cells, blood vessels Endothelial cells and interstitial cells. However, no expression of IL-6 mRNA was detected in 3 normal controls. IL-6mRNA expression in renal tissue of patients with lupus nephritis increased, and the high expression of IL-6mRNA and serum ANA levels and kidney IgG, C3 deposition may be related. Tip IL-6 in the pathogenesis of lupus nephritis may have an important pathological role.