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目的:探讨血清高尔基体蛋白(GP73)联合Dickkopf-1、甲胎蛋白(AFP)对肝细胞癌的诊断价值。方法:收集2014年9月至2016年9月我院收治的117例肝细胞癌患者(肝细胞癌组)、80例乙型病毒性肝炎患者(肝炎组),以及随机选取的80例健康体检者(对照组)为研究对象,采用酶联免疫吸附法(ELISA)测定各组对象的血清GP73、Dickkopf-1、AFP水平,并分析其与临床病理特征之间的关系,分析GP73、Dickkopf-1、AFP联合对肝细胞癌的诊断价值。结果:肝细胞癌组血清GP73、Dickkopf-1、AFP水平高于肝炎组、对照组,且肝炎组血清AFP水平高于对照组,差异有统计学意义(P<0.05)。Child Pugh分级中B~C级肝细胞癌患者GP73、Dickkopf-1、AFP水平高于A级,差异有统计学意义(P<0.05),不同分化程度、肿瘤直径、肿瘤数目患者的GP73、Dickkopf-1、AFP水平差异无统计学意义(P>0.05)。GP73+Dickkopf-1+AFP对肝细胞癌诊断的灵敏度、特异性、阳性预测值、阴性预测值、准确率均高于GP73、Dickkopf-1、AFP的诊断结果,差异有统计学意义(P<0.05)。结论:血清GP73、Dickkopf-1、AFP在肝细胞癌中呈高表达水平,三者联合可明显提高对肝细胞癌的诊断价值,临床有重要的参考价值。
Objective: To investigate the diagnostic value of serum prokaryotic protein (GP73) combined with Dickkopf-1 and AFP in hepatocellular carcinoma. Methods: Totally 117 hepatocellular carcinoma patients (hepatocellular carcinoma), 80 hepatitis B patients (hepatitis group) and 80 healthy controls were collected from September 2014 to September 2016 in our hospital. (Control group). The levels of GP73, Dickkopf-1 and AFP in each group were determined by enzyme-linked immunosorbent assay (ELISA), and the relationship between GP73 and Dickkopf-1 and AFP was analyzed. 1, AFP combined diagnosis of hepatocellular carcinoma. Results: The serum levels of GP73, Dickkopf-1 and AFP in hepatocellular carcinoma group were higher than those in hepatitis group and control group. The serum AFP level in hepatitis group was higher than that in control group (P <0.05). The levels of GP73, Dickkopf-1 and AFP in Child Pugh grade B ~ C hepatocellular carcinoma were significantly higher than those in Grade A (P <0.05). The GP73, Dickkopf -1, AFP level difference was not statistically significant (P> 0.05). The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of GP73 + Dickkopf-1 + AFP in diagnosing hepatocellular carcinoma were all higher than those of GP73, Dickkopf-1 and AFP (P < 0.05). Conclusion: Serum GP73, Dickkopf-1 and AFP are highly expressed in hepatocellular carcinoma. The combination of the three can significantly improve the diagnostic value of hepatocellular carcinoma, which has important clinical value.