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[目的]探讨人体外淋巴细胞氧化损伤模型的建立。[方法]抽取10例健康志愿者的外周血,分离淋巴细胞,建立暴露于低浓度乙醇下淋巴细胞氧化损伤模型,并检测暴露于不同浓度乙醇下淋巴细胞的氧化损伤标记物-8-羟基鸟嘌呤(8-OH-dG)进行验证。[结果]分离的10例志愿者外周血淋巴细胞活性大于90%,暴露于乙醇的浓度与淋巴细胞生长抑制成显著正相关(r=0.993,P﹤0.001)。不同浓度乙醇(200mmol/L、100mmol/L和50mmol/L)对淋巴细胞染毒2h后,8-Oh-dG水平显著高于对照组(P﹤0.001),随着乙醇浓度的增加,8-OhdG水平越高。[结论]50mmol/L乙醇作用淋巴细胞2h可以建立体外淋巴细胞氧化损伤模型。
[Objective] To explore the establishment of human peripheral lymphocyte oxidative damage model. [Methods] Peripheral blood of 10 healthy volunteers was collected and lymphocytes were isolated. A model of oxidative damage of lymphocytes exposed to low concentration of ethanol was established and the oxidative damage markers of -8-hydroxy Purine (8-OH-dG) was validated. [Results] The activity of peripheral blood lymphocytes in 10 isolated volunteers was more than 90%. There was a significant positive correlation (r = 0.993, P <0.001) between the ethanol exposure and the growth of lymphocytes. The levels of 8-Oh-dG in lymphocytes were significantly increased after treated with different concentrations of ethanol (200mmol / L, 100mmol / L and 50mmol / L) for 2h (P <0.001). With the increase of ethanol concentration, OhdG the higher the level. [Conclusion] The model of oxidative damage of lymphocytes can be established in vitro by 50mmol / L ethanol-treated lymphocytes for 2h.