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目前,头发中钙的测定一般采用原子吸收法,光度显色法对头发中钙的测定报道甚少。本文在前人工作的基础上对头发中钙的测定方法进行了试验,并测定了上千个样品,通过试验,我们认为此方法简便、快速、灵敏、结果准确可靠,特别适用于基层单位大批样品分析。 1 试验部分1.1 仪器与试剂 721分光光度计混合酸:高氯酸(AR):硝酸(AR)=1:8 显色试剂:称取1.6g 8-羟基喹啉于小烧杯中,加浓盐酸1.2ml,重蒸水10ml溶解后,再称取聚乙烯吡咯啉酮(PVP)2.0g,甲基百里香酚蓝络合物(MTB)80mg,
At present, the determination of calcium in hair is generally based on atomic absorption spectrometry, photometric colorimetric method for the determination of calcium in the hair little. Based on the work of predecessors, this paper tested the determination of calcium in hair and measured thousands of samples. Through the experiment, we think this method is simple, rapid and sensitive, the results are accurate and reliable, especially suitable for a large number of grassroots units Sample analysis. 1 Experimental 1.1 Instruments and reagents 721 spectrophotometer mixed acid: perchloric acid (AR): nitric acid (AR) = 1: 8 chromogenic reagent: Weigh 1.6g 8-hydroxyquinoline in a small beaker, add concentrated hydrochloric acid 1.2ml, redissolved water 10ml dissolved, then weighed polyvinylpyrrolidone (PVP) 2.0g, methyl thymol blue (MTB) 80mg,