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目的建立天然抗角蛋白自身抗体(AK auto Ab)的转基因小鼠模型。方法将转基因表达质粒显微注射法转入CBA×C57BL/6小鼠受精卵,导入假孕雌鼠输卵管后培育子代小鼠;PCR鉴定子代小鼠基因型;ELISA法分析转基因小鼠体内AK auto Ab水平的变化。结果共获得12系转基因阳性的 Founder小鼠,其中9系传至第3代;3系转基因小鼠体内的AK auto Ab水平显著增高。结论成功建立 AK auto Ab的转基因小鼠,获得了血清AK auto Ab水平增高的动物模型。
Objective To establish a transgenic mouse model of natural keratin autoantibody (AK auto Ab). Methods Transgenic expression plasmids were transfected into fertilized eggs of CBA × C57BL / 6 mice by microinjection and introduced into the oviduct of pseudopregnant females to develop offspring mice. PCR was used to identify the offspring mouse genotypes. AK auto Ab level changes. Results A total of 12 Founder mice with positive transgenic lines were obtained. Among them, 9 passages were passaged to passage 3; AK auto Ab levels in 3 transgenic mice were significantly increased. Conclusion The transgenic mice with AK auto Ab were successfully established and the animal models with elevated serum levels of AK auto Ab were obtained.