对克隆的 WSSV基因组 Xba 1.8kb片段进行序列测定 ,根据所测定的核酸序列 ,通过计算机软件分析 ,设计出一对 PCR引物。所设计的 PCR引物能从纯化 WSSV及患白斑症的对虾组织中扩增出长为 92 1bp的目的 DNA片段 ,并且能检测出 0 .2～ 0 .4μg病虾肌肉组织中的 WSSV。健康对虾、感染MBV的斑节对虾仔虾及 SINPV的扩增结果均为阴性。表明所建立的 WSSV PCR检测法灵敏而且特异。 The cloned WSSV genome Xba 1.8kb fragment was sequenced, and based on the determined nucleic acid sequence, a pair of PCR primers was designed by computer software analysis. The PCR primers were designed to amplify the target DNA fragment of 92 1bp from WSSV and white spot-infected shrimp tissues, and detect WSSV from 0.2 to 0.4 μg in shrimp muscle tissues. Health shrimp, MBV infection of Penaeus monodon and SINPV amplification results were negative. The established WSSV PCR detection method is sensitive and specific.