论文部分内容阅读
采用自行研制成的功能性抗人CD40单克隆抗体、转导CD32的L细胞(LCD32)和细胞因子建立体外研究人B淋巴细胞增殖、生长和分化的CD40系统。方法:①流式细胞仪分析CD40分子的表达;②在CD40激发型单抗(克隆5C11)与LCD32共培养中,加入IL-4及其它细胞因子,组成CD40培养系统,观察人扁桃体来源B细胞的体外生存和增殖等生物学效应。结果:①CD40分子表达于人外周血、扁桃体来源的B细胞及人多发性骨髓瘤(MM)细胞株XG2和人B淋巴瘤细胞株Daudi;②用CD40激发型单抗(克隆5C11和2G11)与LCD32细胞联合IL-4能使静止期B细胞体外长期生存和增殖。表明用激发型CD40单抗5C11和2G11建立的CD40培养体系,能使B细胞长期生存和增殖,为体外研究B细胞提供了有效的手段。
CD40 system was established to study the proliferation, growth and differentiation of human B lymphocytes in vitro using functional anti-human CD40 monoclonal antibody developed by ourselves, transducing CD32 L cells (LCD32) and cytokines. Methods: (1) The expression of CD40 was analyzed by flow cytometry; (2) IL-4 and other cytokines were added into the co-culture of CD40 monoclonal antibody (clone 5C11) and LCD32 to form CD40 culture system; In vitro biological effects such as survival and proliferation. Results ①CD40 expression in human peripheral blood, tonsil-derived B cells and human multiple myeloma (MM) cell line XG2 and human B lymphoma cell line Daudi; ② CD40-activated monoclonal antibody (clone 5C11 and 2G11) and LCD32 cells combined with IL-4 can make stationary B cells in vitro long-term survival and proliferation. The results showed that the CD40 culture system established by the stimulated CD40 McAbs 5C11 and 2G11 can make B cells survive and proliferate for a long time and provide an effective method for studying B cells in vitro.