目的探究龈沟液中是否存在白细胞介素８（ｉｎｔｅｒｌｅｕｋｉｎ８，ＩＬ８）的抑制因子。方法（１）将１３例成人牙周炎的１４份及８例牙周健康者的９份龈沟液样本各自一分为二，１／２加入丝氨酸蛋白酶抑制剂──苯甲基磺酰氟，另１／２加入等量的磷酸盐缓冲液作为对照。ＥＬＩＳＡ法检测各样本中的ＩＬ８含量。（２）对１５例成人牙周炎的４１份龈沟液样本，用间接ＥＬＩＳＡ法测定龈沟液中的ＩＬ８自身抗体（ＩｇＧ）。结果（１）加入酶抑制剂的龈沟液中ＩＬ８的检出量（３．０１ｍｇ／Ｌ±５．７９ｍｇ／Ｌ）显著高于对照组（０．０５ｍｇ／Ｌ±０．１５ｍｇ／Ｌ），Ｐ＜０．００１。（２）龈沟液中ＩＬ８自身抗体水平高于阴性对照标准差３倍。龈沟液中加入大肠杆菌超声粉碎液对此检测结果无明显影响。结论龈沟液中存在能降解ＩＬ８的丝氨酸蛋白酶；成人牙周炎的龈沟液中存在ＩＬ８自身抗体 Objective To investigate the presence of interleukin8 (IL8) inhibitor of gingival crevicular fluid. Methods: (1) 14 samples of 13 adult periodontitis and 8 samples of 8 periodontal healthy persons were divided into two groups respectively. One-half of them were treated with serine protease inhibitor, phenylmethylsulfonyl fluoride , And the other 1/2 added the same amount of phosphate buffer as a control. ELISA method to detect the content of IL 8 in each sample. (2) 41 gingival crevicular fluid samples of 15 cases of adult periodontitis were measured by indirect ELISA method in the GCF of IL  8 autoantibodies (IgG). Results (1) The detection of IL8 in GCF with enzyme inhibitor (3.01mg / L ± 5.79mg / L) was significantly higher than that of the control group (0.05mg / L ± 0.15mg / L ), P <0.001. (2) The level of IL8 autoantibody in gingival crevicular fluid was 3 times higher than that of negative control. Gingival crevicular fluid by adding E. coli ultrasonic pulverized liquid test results no significant effect. Conclusion gingival crevicular fluid exists in the degradation of IL 8 serine protease; adult periodontitis in the gingival crevicular fluid IL 8 autoantibodies exist