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目的制备酪氨酸激酶受体anexelekto(AXL)单克隆抗体(mAb),建立可溶型AXL(sAXL)的ELISA试剂盒并对肾综合征出血热患者血浆sAXL含量进行检测。方法采用商品化的AXL抗原作为免疫原,常规方法制备小鼠源性mAb,间接ELISA检测小鼠腹水效价,阵距法配对建立sAXL夹心ELISA试剂盒。结果成功制备了检测sAXL的ELISA试剂盒,肾综合征出血热患者血浆中sAXL水平明显高于正常人水平。结论制备了酪氨酸激酶受体AXL的mAb并成功制备了sAXL的ELISA试剂盒,适用于人血浆中sAXL的定量检测。
Objective To prepare anexelekto (AXL) monoclonal antibody (mAb) for tyrosine kinase receptor, and to establish a soluble AXL (sAXL) ELISA kit for the determination of plasma sAXL in patients with hemorrhagic fever with renal syndrome. Methods Commercially available AXL antigens were used as immunogens. Mouse-derived mAbs were prepared by conventional methods. The ascites titer of mice was determined by indirect ELISA. The sAXL sandwich ELISA kit was established by the array method. Results The sAXL ELISA kit was successfully prepared. The level of sAXL in plasma of patients with hemorrhagic fever with renal syndrome was significantly higher than that of normal people. Conclusion The mAb of tyrosine kinase receptor AXL was prepared and the sAXL ELISA kit was successfully prepared for the quantitative detection of sAXL in human plasma.