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[目的]研究长期低剂量铜暴露对小鼠海马组织铜含量、铜代谢相关酶及氧化应激的影响,为深入研究低剂量铜暴露的神经毒性作用提供基础数据。[方法]选择野生型小鼠,随机分为对照组和试验组对照组小鼠饮用蒸馏水,试验组在饮用水中添加0.13 mg/L氯化铜,连续饮用3个月,研究长期低剂量铜暴露对小鼠海马组织中铜含量、铜代谢相关酶表达及氧化应激的影响。[结果]与对照组相比,试验组小鼠海马中的自由铜及结合铜含量均显著升高,而铁、锌、钙离子含量在海马中均无显著差异;与对照组相比,试验组小鼠海马组织中铜蓝结合蛋白表达显著降低,蛋白硝基酪氨酸水平显著升高,8-羟基脱氧鸟嘌呤荧光表达水平显著升高。[结论]长期超低剂量铜暴露可导致铜在脑组织中聚集,但不影响铁、锌、钙和镁的含量;长期低剂量铜暴露可导致氧化应激。
[Objective] To investigate the effects of long-term low-dose copper exposure on copper content, copper-related enzymes and oxidative stress in hippocampal tissue of mice and provide basic data for further study on the neurotoxicity of low-dose copper exposure. [Methods] Wild-type mice were selected and randomly divided into control group and control group mice drinking distilled water. The experimental group added 0.13 mg / L cupric chloride to drinking water continuously for 3 months to study the effect of chronic low-dose copper Effects of exposure on copper content, expression of copper metabolism related enzymes and oxidative stress in hippocampus of mice. [Results] Compared with the control group, the contents of free copper and copper in the hippocampus of the experimental group were significantly increased, while there was no significant difference in the content of iron, zinc and calcium in the hippocampus. Compared with the control group, the test The expression of copper-blue-binding protein in hippocampus of mice was significantly decreased, the level of nitrotyrosine protein was significantly increased, and the fluorescence intensity of 8-hydroxy-deoxyguanosine was significantly increased. [Conclusion] Long-term ultra-low dose exposure of copper can lead to the accumulation of copper in brain tissue, but does not affect the content of iron, zinc, calcium and magnesium; long-term low-dose copper exposure can lead to oxidative stress.