论文部分内容阅读
目的:探讨喉鳞状细胞癌(LSCC)瘤内淋巴管生成与淋巴转移的关系,为LSCC转移和预后的判定以及治疗提供一定的理论依据。方法:通过免疫病理学方法计数LSCC组织中淋巴管密度,通过分子生物学方法检测中血管内皮生长因子-C(VEGF-C)的表达,统计学方法对淋巴管密度(LVD)和患者性别、肿瘤TNM分期、VEGF-C表达和肿瘤分化程度进行分析,得出相关性结论。结果:喉癌瘤体内有新生淋巴管存在,喉癌组织中VEGF-CmRNA的平均水平与正常组织之间存在明显差异(P<0.05),瘤组织比正常组织高4～5倍,而且喉癌组织内VEGF-C的水平与瘤内LVD高低之间差异有统计学意义(P<0.05),LVD在患者的性别,临床T分期以及肿瘤的分化程度各组之间均差异无统计学意义(均P>0.05)。而与N分期内差异有统计学意义(P<0.05)。结论:LSCC瘤组织中存在小的增殖性淋巴管且LSCC瘤内LVD的增高与肿瘤淋巴转移有关;VEGF-CmRNA在LSCC组织中的表达要比正常对照组织高,且VEGF-CmRNA的高表达可能会通过促进瘤内淋巴管的增生来促进喉癌的淋巴转移。
Objective: To investigate the relationship between lymphangiogenesis and lymphatic metastasis in laryngeal squamous cell carcinoma (LSCC) and to provide some theoretical evidences for the metastasis and prognosis of LSCC. Methods: The lymphatic vessel density in LSCC tissues was counted by immunopathological method. The expression of VEGF-C was detected by molecular biology method. The relationship between lymphatic vessel density (LVD) and sex, Tumor TNM staging, VEGF-C expression and tumor differentiation were analyzed, the conclusion was drawn. Results: Lymphatic vessels were found in laryngeal carcinoma. The average level of VEGF-C mRNA in laryngeal carcinoma tissues was significantly different from that of normal tissues (P <0.05). The tumor tissue was 4 to 5 times higher than normal tissues, The difference between the level of VEGF-C and the level of intratumoral LVD was statistically significant (P <0.05). There was no significant difference in the gender, clinical stage and tumor differentiation between the two groups All P> 0.05). However, there was significant difference with N stage (P <0.05). CONCLUSIONS: Small proliferative lymphangiomas exist in LSCC and the increase of LVD in LSCC is related to tumor lymphatic metastasis. The expression of VEGF-C mRNA in LSCC is higher than that in normal control, and the high expression of VEGF-C mRNA may be Lymphatic metastasis of laryngeal cancer can be promoted by promoting the proliferation of lymphatic vessels in the tumor.