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目的 :探讨TGF β对胎兔颅骨成骨样细胞体外成骨作用。方法 :显微相差动态观察、细胞增殖计数、ALP活性测定及骨结节计数观察TGF β体外成骨作用。结果 :TGF β对该细胞的增殖 ,低剂量有促进作用 ,高剂量有抑制作用。TGF β(在 3~ 7d)对ALP活性有促进作用 ,并呈一定的时效和量效关系。TGF β低剂量对骨结节形成有促进作用 ,高剂量时为强有力的抑制作用 ,与剂量有相关关系。结论 :TGF β对胎兔颅骨成骨样细胞体外成骨表现为双相作用。
OBJECTIVE: To investigate the in vitro osteogenesis effect of TGF-β on fetal skull-like cells. Methods: The in vitro osteogenesis of TGF-β was observed by dynamic observation of microdissection, counting of cell proliferation, determination of ALP activity and counting of bone nodules. Results: TGF β could promote the proliferation and low dose of the cells, and inhibit the high dose of TGF β. TGFβ (at 3 ~ 7d) promoted the activity of ALP, and showed a certain aging and dose-response relationship. The low dose of TGF beta can promote the formation of bone nodules, which is a strong inhibitory effect at high dosage and has a correlation with the dose. Conclusion: TGF β can induce osteoblast-like cells in vitro to exhibit biphasic osteogenesis.